Volume 6.18 | May 12

Hematopoiesis News 6.18 May 12, 2015
Hematopoiesis News
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Inducible Gata1 Suppression Expands Megakaryocyte-Erythroid Progenitors from Embryonic Stem Cells
Investigators engineered embryonic stem cells from WT mice to express a doxycycline-regulated (dox-regulated) shRNA that targets Gata1 transcripts for degradation. Differentiation of these cells in the presence of dox and thrombopoietin resulted in an exponential (at least 1013-fold) expansion of immature hematopoietic progenitors. [J Clin Invest] Full Article | Press Release
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PUBLICATIONS (Ranked by impact factor of the journal)
Deficiency in WT1-Targeting MicroRNA-125a Leads to Myeloid Malignancies and Urogenital Abnormalities
MicroRNA-125a (miR-125a) was identified as a miRNA that suppressed WT1 expression via binding to the WT1-3’UTR. miR-125a knockout mice overexpressed WT1, developed myeloproliferative disorder characterized by expansion of myeloid cells in bone marrow, spleen and peripheral blood, and displayed urogenital abnormalities. [Oncogene] Abstract

RCAD/Ufl1, a Ufm1 E3 Ligase, Is Essential for Hematopoietic Stem Cell Function and Murine Hematopoiesis
Researchers report that RCAD/Ufl1 is essential for embryonic development, hematopoietic stem cell survival and erythroid differentiation. Both germ-line and somatic deletion of RCAD/Ufl1 impaired hematopoietic development, resulting in severe anemia, cytopenia and ultimately animal death. [Cell Death Differ] Abstract

Age-Related Increase of EED Expression in Early Hematopoietic Progenitor Cells Is Associated with Global Increase of the Histone Modification H3K27me3
EED is expressed at comparable amounts in neonatal and adult multipotent hematopoietic stem and progenitor cells (HSPCs), early adult lineage committed progenitors of the lympho-myeloid and erythro-myeloid lineages expressed higher EED amounts than neonatal HPCs. Scientists demonstrated that EED overexpression directly led to higher H3K27me3 levels, a repressive histone modification that is mediated by the polycomb repressive complex 2 complex. [Stem Cells Dev] Abstract

Human TM9SF4 Is a New Gene Down-Regulated by Hypoxia and Involved in Cell Adhesion of Leukemic Cells
Investigators analyzed the expression and regulation of TM9SF4 in normal and leukemic cells and identified TM9SF4 as a gene highly expressed in human quiescent CD34+ hematopoietic progenitor cells (HPCs), regulated during monocytic and granulocytic differentiation of HPCs, both lineages giving rise to mature myeloid cells involved in adhesion, phagocytosis and immunity. [PLoS One] Full Article

Murine Hematopoietic Stem Cell Dormancy Controlled by Induction of a Novel Short Form of PSF1 by Histone Deacetylase Inhibitors
Scientists used trichostatin A, a histone deacetylase inhibitor, to enforce histone acetylation and showed that this suppresses cell cycle entry by dormant hematopoietic stem cells. [Exp Cell Res] Abstract


Allogeneic Cell Transplant Expands Bone Marrow Distribution by Colonizing Previously Abandoned Areas: An FDG PET/CT Analysis
Investigators applied a computational quantification software application to hybrid FDG PET/CT images to assess activity and distribution of the hematopoietic system throughout the whole skeleton of recently transplanted patients. [Blood] Abstract

Recipient Expression of Ligands for Donor Inhibitory KIRs Enhances NK-Cell Function, to Control Leukemic Relapse after Haploidentical Transplantation
Researchers compared the functionality of reconstituting natural killer (NK) cells, based on CD107α expression and interferon-γsecretion, in a cohort of 29 patients that expressed or lacked class-I HLAs for donor inhibitory KIRs following T-cell-replete haploidentical stem cell transplantation. [Eur J Immunol] Abstract

Long-Term Outcome of HLA-Haploidentical Hematopoietic Stem Cell Transplantation without In Vitro T-Cell Depletion Based on an FBCA Conditioning Regimen for Hematologic Malignancies
One hundred and five consecutive patients with hematologic malignancies who underwent G-CSF-primed peripheral blood haploidentical hematopoietic stem cell transplantation without in vitro T-cell depletion in a single center were reported. [Bone Marrow Transplant] Abstract

Live Webinar: New Tools for Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
Non-Steady-State Hematopoiesis Regulated by the C/EBPβ Transcription Factor
The BCR-ABL fusion protein activates emergency-specific pathway of granulopoiesis by up-regulating C/EBPβ. This in turn causes chronic phase chronic myeloid leukemia, which is characterized by myeloid expansion. C/EBPβ also plays a role in other hematological malignancies of both myeloid- and lymphoid lineage-origin. [Cancer Sci] Abstract

Visit our reviews page to see a complete list of reviews in the hematopoiesis research field.

Target Cancer Metabolism
Dana-Farber/Boston Children’s Opens Immunotherapy Trial for Children with Leukemia
Dana-Farber/Boston Children’s Cancer and Blood Disorders Center has joined a clinical trial of immunotherapy for children with relapsed or treatment-resistant acute lymphoblastic leukemia. [Dana-Farber Cancer Institute] Press Release

Pluristem Granted European Patent for Use of Cells to Help Treat Damaged Bone Marrow
Pluristem Therapeutics Inc. announced that it has been issued Patent No. EP2366775B1, titled “Methods for Cell Expansion and Uses of Cells and Conditioned Media Produced Thereby for Therapy”, by the European Patent Office. Pluristem’s therapeutic cells are designed to promote the success of hematopoietic stem cell transplantation, which is used to treat patients with dysfunctional bone marrow. [Pluristem Therapeutics Inc.] Press Release

Bellicum Pharmaceuticals Leases Space for Pilot Manufacturing Facility
Bellicum Pharmaceuticals, Inc. announced that it has entered into a lease agreement to enable in-house cell therapy manufacturing to supply clinical trials. The additional 27,000 square feet will be located in the company’s current headquarters building adjacent to the Texas Medical Center in Houston. [Bellicum Pharmaceuticals, Inc.] Press Release

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NEW 25th Annual Mayo Clinic Hematology/Oncology Reviews
July 29-August 1, 2015
Amelia Island, United States

Visit our events page to see a complete list of events in the hematopoiesis community.
NEW Postdoctoral Position – Epigenetic Regulation of Hematopoiesis and Leukemia (Karolinska Institute)

Principal Scientist – Hematopoietic Products (STEMCELL Technologies Inc.)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

Postdoctoral Scientist – Hematopoiesis (University of Oxford)

Postdoctoral Positions – Regulation of Normal and Malignant Hematopoiesis (Karolinska Institute)

Postdoctoral Researcher – Hematopoietic Stem Cell Physiology and Pathophysiology (International Research Center for Medical Sciences)

Postdoctoral Associate – Epigenetic Regulation of Normal and Leukemic Stem Cells (Sanford-Burnham Medical Research Institute)

Postdoctoral Fellow – Hematopoietic Stem Cell Biology (University of Leuven)

Postdoctoral Fellow – Leukemia, Stem Cells and Development (Josep Carreras Leukemia Research Institute)

Postdoctoral Fellow – Hematopoietic Stem Cell Research (Lund University)

Postdoctoral Research Fellow – Stem Cell/Hematopoiesis Research (BloodCenter of Wisconsin)

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