Volume 5.21 | Jun 3

Hematopoiesis News 5.21 June 3, 2014
Hematopoiesis News
     In this issue: Publications | Reviews | Industry News | Policy News | Events | Jobs
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TOP STORY
Targeted Genome Editing in Human Repopulating Hematopoietic Stem Cells
The authors show that poor permissiveness to gene transfer and limited proficiency of the homology-directed DNA repair pathway constrain gene targeting in human hematopoietic stem cells (HSCs). By tailoring delivery platforms and culture conditions they overcame these barriers and provide stringent evidence of targeted integration in human HSCs by long-term multilineage repopulation of transplanted mice. [Nature]
Abstract | Press Release
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PUBLICATIONS (Ranked by impact factor of the journal)
LABORATORY RESEARCH

Identification of Erythroferrone as an Erythroid Regulator of Iron Metabolism
Investigators identified a new hormone, erythroferrone (ERFE) that mediates hepcidin suppression during stress erythropoiesis. ERFE is produced by erythroblasts in response to erythropoietin. [Nat Genet]
Abstract | Press Release

PADI4 Acts as a Coactivator of Tal1 by Counteracting Repressive Histone Arginine Methylation
The transcription factor Tal1 is a critical activator or repressor of gene expression in hematopoiesis and leukemia. The authors show that Tal1 interacts with the peptidylarginine deiminase IV (PADI4). They demonstrate that PADI4 can act as an epigenetic coactivator through influencing H3R2me2a. [Nat Commun]
Full Article

In Utero Depletion of Fetal Hematopoietic Stem Cells Improves Engraftment after Neonatal Transplantation in Mice
Scientists used an antibody against the murine c-Kit receptor (ACK2) to deplete fetal host hematopoietic stem cells and increase space within the hematopoietic niche for donor cell engraftment. Fetal mice were injected with ACK2 on E13.5-14.5 and surviving pups were transplanted with congenic hematopoietic cells on day of life one. [Blood] Abstract

Dissection of Vertebrate Hematopoiesis Using Zebrafish Thrombopoietin
To identify and prospectively isolate thrombocytic/erythroid progenitors, investigators identified, cloned, and generated recombinant zebrafish thrombopoietin (Tpo). Tpo mRNA expanded itga2b:GFP+ thrombocytes as well as hematopoietic stem and progenitor cells in the zebrafish embryo. [Blood] Abstract

The Cebpa +37-kb Enhancer Directs Transgene Expression to Myeloid Progenitors and to Long-Term Hematopoietic Stem Cells
FACS analysis, in vitro colony assays, and in vivo competitive and secondary transplantation revealed that myeloid but not MEPs or lymphoid progenitors and also functional LT-HSCs are found almost exclusively in the Cebpa-hCD4+ compared with hCD4 marrow population. [J Leukoc Biol] Abstract

Low Dose Insulin Like Growth Factor Binding Proteins 1 and 2 and Angiopoietin Like Protein 3 Coordinately Stimulate Ex Vivo Expansion of Human Umbilical Cord Blood Hematopoietic Stem Cells as Assayed in NOD/SCID Gamma Null Mice
In order to determine the optimal dose and combination of insulin like growth factors (IGFs), IGF binding proteins (IGFBPs) and angiopoietin like proteins (ANGPTLs), serial dilution and full permutation of IGFBP1, IGFBP2, IGF2 and ANGPTL3 were applied on a cryopreserved umbilical cord blood mononuclear cells ex vivo expansion system. [Stem Cell Res Ther] Abstract | Full Article

Dppa5 Improves Hematopoietic Stem Cell Activity by Reducing Endoplasmic Reticulum Stress
Scientists demonstrate that developmental pluripotency-associated 5 (Dppa5) is a regulator of hematopoietic stem cells that critically governs reconstitution capacity after bone marrow transplantation. [Cell Rep]
Full Article | Graphical Abstract

CLINICAL RESEARCH

Ibrutinib versus Ofatumumab in Previously Treated Chronic Lymphoid Leukemia
In this multicenter, open-label, Phase III study, researchers randomly assigned 391 patients with relapsed or refractory chronic lymphoid leukemia or small lymphocytic lymphoma to receive daily ibrutinib or the anti-CD20 antibody ofatumumab. [N Engl J Med] Abstract | Press Release

Plerixafor plus Pegfilgrastim Is a Safe, Effective Mobilization Regimen for Poor or Adequate Mobilizers of Hematopoietic Stem and Progenitor Cells: A Phase I Clinical Trial
The safety, kinetics and efficacy of plerixafor+pegfilgrastim for hematopoietic stem and progenitor cell mobilization are poorly understood. Investigators treated 12 study patients with pegfilgrastim and plerixafor. [Bone Marrow Transplant] Abstract

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REVIEWS
Role of Rho Kinases in Abnormal and Normal Hematopoiesis
In blood cells, emerging data suggest that Rho kinase plays an essential role in negatively regulating inflammatory and erythropoietic stress and positively regulates the growth and survival of leukemic cells. [Curr Opin Hematol] Abstract

Visit our reviews page to see a complete list of reviews in the hematopoiesis research field.
 
INDUSTRY NEWS
Sangamo BioSciences and City of Hope Granted Strategic Partnership Award from California Institute for Regenerative Medicine to Support Clinical Trial of Stem-Cell Based ZFP Therapeutic® for HIV/AIDS
Sangamo BioSciences, Inc. announced that the California Institute for Regenerative Medicine has granted a $5.6 million Strategic Partnership Award to fund clinical studies at City of Hope to develop a potentially curative ZFP Therapeutic for HIV/AIDS based on the application of Sangamo’s zinc finger nuclease genome-editing technology in hematopoietic stem/progenitor cells. [Sangamo BioSciences, Inc.] Press Release

Aastrom Completes Acquisition of Sanofi’s Cell Therapy and Regenerative Medicine Business
Aastrom Biosciences, Inc. announced that the company has completed its acquisition of Sanofi’s cell therapy and regenerative medicine business unit. [Aastrom Biosciences, Inc.] Press Release

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POLICY NEWS
National Institutes of Health (United States)

Food and Drug Administration (United States)

Center for Biologics Evaluation and Research (United States)

European Medicines Agency (European Union)

Medicines and Healthcare Products Regulatory Agency (United Kingdom)

Therapeutic Goods Administration (Australia)
 
EVENTS
NEW 13th Annual American Association for Cancer Research (AACR) International Conference on Frontiers in Cancer Prevention Research
September 28 – October 1, 2014
New Orleans, United States

Visit our events page to see a complete list of events in the hematopoiesis research community.
 
JOB OPPORTUNITIES
NEW Postdoctoral Position/Clinical Pharmacist – Hematopoiesis and Laboratory for Cell Therapy (Sanquin)

Clinical HSCT Faculty Position – Pediatric Hematology-Oncology (University of Wisconsin School of Medicine)

Postdoctoral Research Scientist – Stem Cell Biology and Cellular Immunology (The Anthony Nolan Trust)

Postdoctoral Fellow – Mammalian Hematopoietic and Erythroid Development (Icahn School of Medicine at Mount Sinai)

Postdoctoral Fellow – Regulation of Cytokine Receptor Signaling in Hematopoietic Stem Cells (University of Pennsylvania School of Medicine)

Postdoctoral Positions – New Models of Hematopoiesis and Hematopoietic Disorders (Icahn School of Medicine at Mount Sinai)

Postdoctoral Researcher – Cancer Genomics (University of Chicago)

Postdoctoral Associate – Hematopoietic Differentiation (Northwestern University Feinberg School of Medicine)

Postdoctoral Fellowship – Hematology, Immunology, Cancer Therapeutics (Nationwide Children’s Hospital)

Scientist – Immunology/Cell Separation (STEMCELL Technologies Inc.)

Research Technologist – hPSC (STEMCELL Technologies Inc.)

Research Associate – Cell Separation (STEMCELL Technologies Inc.)

Research Technologist – Pluripotent Stem Cells (STEMCELL Technologies Inc.)

Research Technologist – PSC Biology and Bioengineering (STEMCELL Technologies Inc.)


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